medium · Enhanced ACT science

In an enzyme experiment, the rate of product formation (in μ mol/min) was measured at five substrate concentrations, at both 25°C and 37°C:

| Substrate (mM) | Rate 25°C | Rate 37°C |

|:---|:---|:---|

| 2 | 8 | 14 |

| 4 | 14 | 24 |

| 8 | 21 | 38 |

| 16 | 25 | 47 |

| 32 | 26 | 49 |

A student claims that doubling substrate concentration always at least doubles the reaction rate. Do the data support this claim?

  1. Yes, since every rate value recorded at 37 degrees Celsius is larger than the matching value seen at 25 degrees.
  2. No, because at higher substrate concentrations the rate increases by progressively smaller amounts and levels off.
  3. Yes, because the measured reaction rate rises every single time the substrate concentration is raised any further at all.
  4. No, because the reaction rate clearly and steadily decreases once substrate concentration rises past 16 mM in this trial.

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