hard · Act science
In an experiment on enzyme activity, the rate of a reaction (in arbitrary units) was measured as substrate concentration increased while enzyme amount was held constant: Substrate (mM): 1, 2, 4, 8, 16, 32 Rate: 10, 18, 30, 40, 45, 46 Which conclusion is BEST supported by the data?
- Rate increases in direct proportion to substrate concentration across the entire range tested.
- Rate decreases once substrate concentration exceeds 8 mM, indicating substrate inhibition.
- Rate rises steeply at low substrate levels but levels off at high levels, approaching a maximum.
- Doubling the substrate always doubles the rate, so no maximum rate exists.
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