hard · Enhanced ACT science

In an experiment on enzyme activity, the rate of a reaction (in arbitrary units) was measured as substrate concentration increased while enzyme amount was held constant:

| Substrate (mM) | Rate |

| :--- | :--- |

| 1 | 10 |

| 2 | 18 |

| 4 | 30 |

| 8 | 40 |

| 16 | 45 |

| 32 | 46 |

Which conclusion is BEST supported by the data?

  1. Rate increases in direct proportion to substrate concentration across the entire range tested.
  2. Rate decreases once substrate concentration exceeds 8 mM, indicating substrate inhibition.
  3. Rate rises steeply at low substrate levels but levels off at high levels, approaching a maximum.
  4. Doubling the substrate always doubles the rate, so no maximum rate exists.

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