medium · Enhanced ACT science

An experiment measures the rate of an enzyme-catalyzed reaction at different temperatures:

| Temp (^circC) | Rate (units/sec) |

| :--- | :--- |

| 10 | 5 |

| 30 | 25 |

| 50 | 45 |

| 70 | 10 |

Which of the following best explains the sharp decrease in the reaction rate observed at 70^circC?

  1. The reaction reached a state of chemical equilibrium where the forward and reverse rates are equal.
  2. The reactant molecules are moving so fast that they no longer collide with the enzyme.
  3. The high thermal energy caused the enzyme's three-dimensional protein structure to denature.
  4. The enzyme was consumed as a reactant because the temperature exceeded the activation energy.

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