easy · Enhanced ACT science

A scientist measures the reaction rate of a specific enzyme at varying temperatures and records the following data:

| Temp (^circC) | Reaction Rate (units/min) |

| :--- | :--- |

| 10 | 15 |

| 20 | 35 |

| 30 | 70 |

| 40 | 12 |

Which statement best explains the drastic change in reaction rate observed at 40^circC?

  1. The rate decreased because there was not enough O₂ to facilitate the reaction.
  2. Mitochondria stopped producing ATP, causing the enzyme to lose all its energy.
  3. The reaction reached homeostasis and stabilized the rate to prevent cell damage.
  4. The enzyme denatured because its protein structure unfolded at high heat.

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