medium · Enhanced ACT science

A student hypothesized that increasing the concentration of CO_2 would increase the growth of algae. They placed one tank near a window (high CO_2 via a pump) and another tank in a dark closet (low CO_2, no pump). The window tank grew more algae.

Why is this experiment flawed?

  1. The experiment failed to isolate CO_2 as the only variable, as the tanks also differed in their exposure to light and temperature.
  2. Algae growth should be measured by dry mass rather than by observing the color or thickness of the water in the two different tanks.
  3. The student did not specify the species of algae used, which limits the ability of other scientists to replicate the growth conditions.
  4. A pump was used in only one tank, which could have increased the dissolved oxygen levels, acting as a nutrient for the algae growth.

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