medium · LSAT Reading Comprehension

Bio-fluorescence—the absorption of light at one wavelength and its re-emission at a longer, lower-energy wavelength—is widespread in marine life but was long thought to be rare in terrestrial vertebrates. However, a recent survey using ultraviolet (UV) light has revealed that bio-fluorescence is common among many species of amphibians, including frogs and salamanders. In these species, the skin or bones glow bright green or orange when exposed to UV light. Researchers hypothesize that this serves as a communication signal that is visible to other amphibians but hidden from predators, many of whom cannot see the fluorescent wavelengths. Critics, however, suggest that the fluorescence may be a non-functional byproduct of the chemical composition of amphibian skin or a way to protect the animal from UV radiation damage. If the fluorescence is merely a secondary effect of skin chemistry, then it played no role in the evolutionary selection of these species' visual systems.

Which one of the following findings would provide the best evidence for the hypothesis that bio-fluorescence in amphibians is a functional communication signal?

  1. The wavelengths emitted by the glowing skin coincide precisely with the peak sensitivity of the photoreceptors in the eyes of the same species.
  2. Most predators of these amphibians have eyes that are sensitive to a broad band of wavelengths, including the very green and orange light the skin emits.
  3. Day-active amphibian species, exposed to far more sunlight, fluoresce far more strongly under UV than do their strictly nocturnal forest relatives.
  4. The compound responsible for the fluorescence is also, lab tests confirm, highly effective at killing the common skin fungi that infect these amphibians.
  5. Fluorescence is the single and only channel through which any amphibian species could ever conceivably signal or communicate with members of its own kind.

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