medium · Enhanced ACT science

A biologist wants to test if a new vitamin supplement increases the lifespan of fruit flies. He gives the vitamin to 500 male fruit flies and gives no vitamin to 500 female fruit flies.

Which confounding variable is present?

  1. The duration of the fruit flies' lives.
  2. The presence of a separate control group.
  3. The biological sex of the flies.
  4. The total number of fruit flies used.

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