medium · MCAT bio-biochem

According to the central dogma of molecular biology, genetic information is transferred from DNA to RNA to protein.

Which of the following is true regarding the transcription of DNA into mRNA?

  1. RNA polymerase synthesizes mRNA in the 5' rightarrow 3' direction using the template strand.
  2. Transcription occurs solely in the cytoplasm of eukaryotic cells, not the nucleus.
  3. The mature mRNA sequence is identical to that of the DNA template strand, simply with U replacing T.
  4. RNA polymerase, unlike DNA polymerase, requires a short RNA primer laid down by primase to begin synthesis.

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