hard · MCAT chem-phys
A myoglobin solution in a 1.00-cm cuvette has absorbance 0.80 at 410 nm. A researcher dilutes the sample so its concentration is one-quarter of the original, then measures it in a 2.00-cm cuvette.
Assuming Beer's law holds and that the same fraction of stray light (set aside as negligible) applies, what fraction of the incident 410-nm light is transmitted by the diluted sample?
- About 79%, since the doubled path length is assumed to outweigh the quartered concentration
- About 63% (a net absorbance of 0.20, reflecting the quarter-concentration over double the path)
- About 40%, because quartering the concentration while doubling the path length halves the absorbance to 0.40
- About 6.3%, obtained by applying the diluted absorbance of 0.20 to the original transmittance of 16%
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