hard · MCAT chem-phys

Protein folding is often described as a spontaneous process (Δ G < 0) even though it results in a more ordered protein structure (reduced protein entropy).

What explains this thermodynamic favorability?

  1. The increase in entropy of the surrounding water molecules due to the hydrophobic effect.
  2. The process is actually non-spontaneous overall and requires ATP-driven chaperones throughout.
  3. The decrease in enthalpy (Δ H < 0) from breaking hydrogen bonds between protein and water.
  4. New strong covalent peptide bonds form continuously throughout the entire folding process.

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