medium · MCAT bio-biochem

An enzyme-catalyzed reaction follows Michaelis-Menten kinetics.

If a researcher adds a reversible inhibitor that increases the apparent K_M but leaves the V_max unchanged, which of the following describes the interaction between the inhibitor and the enzyme?

  1. The inhibitor binds to an allosteric site regardless of whether the substrate is present.
  2. The inhibitor binds to the active site and prevents substrate binding.
  3. The inhibitor covalently binds to the enzyme, permanently deactivating it.
  4. The inhibitor binds to an allosteric site only after the substrate has bound.

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