medium · MCAT bio-biochem
A protein mixture is separated using isoelectric focusing in a pH gradient ranging from 3 to 10.
If a specific protein has an isoelectric point (pI) of 6.0, where will it stop moving, and what is its net charge at that position?
- It stops at pH 6; net charge is zero.
- It stops at pH 3; net charge is positive.
- It continues to migrate back and forth across the entire gradient.
- It stops at pH 10; net charge is negative.
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