medium · MCAT bio-biochem

An enzyme-catalyzed reaction is observed in the presence of a molecule that increases the K_M but does not alter the V_max of the reaction.

Which of the following best describes the interaction between this molecule and the enzyme?

  1. The molecule permanently alters enzyme structure via covalent modification of a key active-site residue.
  2. The molecule binds to the active site and can be outcompeted by high substrate concentrations.
  3. The molecule binds an allosteric site regardless of whether the substrate is bound.
  4. The molecule binds only to the enzyme-substrate complex, thereby lowering both V_max and K_M.

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